Isolation, Genome Sequencing, Assembly, Annotation And Characterization Of Thermus Sp. Ccb_Us3_Uf1 From Ulu Slim, Perak, Malaysia

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dc.contributor.authorTeh, Beng Soon
dc.date.accessioned2019-10-08T06:25:41Z
dc.date.available2019-10-08T06:25:41Z
dc.date.issued2011-02
dc.description.abstractThermus sp. CCB_US3_UF1, a thermophilic bacterium has been successfully isolated from a hot spring in Ulu Slim, Perak, Malaysia. The genome consists of 2,243,772 bp and a 19,716 bp plasmid. There are 2293 predicted coding sequences (ORFs), 2 rRNA operons, 13 transposase genes, and 48 tRNA genes for all 20 amino acids in the genome. The genome annotation predicts that the tricarboxylic acid (TCA) cycle is complete. All the proteins/enzymes for carbohydrate metabolism pathways are present. Thermus sp. CCB_US3_UF1 employs the non-oxidative pentose phosphate pathway for the biosynthesis of ATP, histidine and coenzymes. All biosynthetic genes for valine, leucine, and isoleucine are present, implying that this bacterium can generate its own essential amino acids needed for growth. Several enzymes of the Calvin cycle were identified, indicating that Thermus sp. CCB_US3_UF1 may be able to fix CO2. However, due to the absence of the key enzyme of this pathway, ribulose 1,5-biphosphate carboxylase/ oxygenase (RUBISCO), alternative carbon-fixation pathways may be utilized. Regulation of encoded genes DnaJ-DnaK-GrpE, GroEL-GroES, repressor HrcA, disulfide bond chaperone of HSP33 family, chaperone HtpG, and small heat shock protein of HSP20 family helps Thermus sp. CCB_US3_UF1 to adapt to a high temperature environment. Proteases such as HslV, HslU, Clp, and Lon are found in Thermus sp.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8981
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectThermus Sp.en_US
dc.subjectCcb_Us3_Uf1en_US
dc.titleIsolation, Genome Sequencing, Assembly, Annotation And Characterization Of Thermus Sp. Ccb_Us3_Uf1 From Ulu Slim, Perak, Malaysiaen_US
dc.typeThesisen_US
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