Quality Of Stored Cord Blood In National Blood Centre Based On Maternal And Neonatal Factors
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Date
2018
Authors
Azmi, Nor Azhani
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Background: Umbilical cord blood (UCB) is an alternative source of allogeneic
haematopoietic stem cells for transplantation. The quality of collected UCB depends on
its haematopoietic content which directly influences the engraftment and the therapeutic
outcome. To ensure the quality of stored UCB, the acceptance criteria were created at
the UCB bank in National Blood Centre (NBC) based on established international
standard, which resulted in a high discard rate. This study aims to determine the
association of maternal and neonatal factors that could influence the quality of
haematopoietic contents of the UCB collections thus guide the choice of donors and
reduce wastage.
Methods/Design: This is a retrospective cross-sectional study performed by reviewing
records of 339 cord blood donations which were successfully cryopreserved in 2014 at
the NBC, Malaysia. The data on stored UCB analysed were the UCB volume, total
nucleated cell (TNC) count, CD34+ cells concentration, and colony forming units
(CFU). The maternal factors analysed were age, race, gravid status, gestational period
and blood group while the neonatal characteristics included gender, neonatal birth
weight and blood group. One-way ANOVA, independent t-test, Pearson correlation, and
single linear regression were used to determine the association between the factors and
the haematopoietic contents of the UCB collected.
Results: Based on the NBC report, 39% (692) of the total UCB collected (1781) in
2014 met the acceptance criteria and stored. Low TNC and low UCB volume accounted
for the highest reasons for UCB discard. Analysis of the maternal and neonatal factors
showed that gestational age has a weak negative correlation with UCB volume
(r= -0.182, p=0.001). While neonatal birth weight, is significant but showed weak
correlation with CB volume (r=0.174, p=0.001) and TNC count/unit (r=0.116,
p=0.034). Haematopoietic contents are all interrelated; the UCB volume with TNC
count/unit (r=0.594, p<0.001), UCB volume with CD34+/unit (r=0.364, p<0.001) and
UCB volume with CFU (r=0.177, p< 0.015).
Conclusions: This study concluded that maternal and neonatal factors were associated
with the UCB haematopoietic contents. The factors identified were gestational age of
the mother and new born weight which had significant association with the UCB
volume and TNC count.
Description
Keywords
Umbilical cord blood, donation, cord blood banking , maternal and neonatal factors