Evaluation Of Andrographis Paniculata, Eurycoma Longifolia And Phyllagathis Rotundifolia For Antimalarial Activity Against Chloroquine And Mefloquine Resistant Malarial Parasites
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Date
2010-07
Authors
Che Husin, Che Nasriyyah
Journal Title
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Volume Title
Publisher
Universiti Sains Malaysia
Abstract
The emergence of drug resistant malaria parasite is one of the major health problems in the world and the search for new treatment is increasingly becoming a priority. Plants remained an important natural source of health remedy for mankind. However, scientific evidence is crucial to support claims of health benefits. The objectives of this study firstly to determine the antimalarial activity of Andrographis paniculata (A. paniculata) against chloroquine (CQ) and mefloquine (MQ) resistant Plasmodium falciparum (P. falciparum) strain and their efficacy in combination with CQ or MQ. Secondly to study the efficacy of A. paniculata and E. longifolia in combination with CQ against CQ resistant Plasmodium berghei (P. berghei). Lastly to determine the effect of water and ethanol extract of Phyllagathis rotundifolia (P. rotundifolia) on P. berghei (CQ and MQ resistant strain). P. falciparum Dd2 strain was cultured and in vitro assays were done with various concentrations of CQ and MQ individually and in combination with various concentrations of A. paniculata. Parasites were labeled with 3H-hypoxanthine and incorporation of radiolabeled isotope was determined. The 50% inhibitory concentration (IC50) values of CQ, MQ, A. paniculata and various combination of CQ-A. paniculata and MQ-A. paniculata were determined by probit analysis and isobologram.
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Keywords
Andrographis Paniculata, Eurycoma Longifolia And Phyllagathis Rotundifolia , Chloroquine And Mefloquine Resistant Malarial Parasites