Molecular Cloning And Functional Characterization Of A Novel Elovl (Elongase) Family From A Freshwater Teleost (Oreochromis Niloticus)
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Date
2017-06
Authors
Han, Wan Yin
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
At present, seven members of ELOVL family (ELOVL1-7) have been
identified in mammals. In terms of function, they are broadly divided into those that
elongate the saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA),
and those that elongate the polyunsaturated fatty acids (PUFA). Recently, a group of
Elovl proteins, termed as Elovl4-like, was discovered from teleost. However, these
proteins have shown low identities and different in term of function with the typical
identified Elovl4 protein. An interest has arisen to investigate whether this group of
Elovl proteins could be a new Elovl family member or as a subfamily of the existing
Elovl member. This study was undertaken to clone and analyse this Elovl gene
sequence (XM_005479121.2) from a freshwater fish, Nile tilapia (Oreochromis
niloticus). Results showed that the sequence of Nile tilapia Elovl elongase revealed
low identities with the other identified Elovl elongase (Elovl1-7) while high identities
with a group of proteins termed Elovl4-like. The results had suggested that this group
of Elovl proteins can be classified as a new member of Elovl family and it is
designated as putative Elovl8. An in-vitro study was carried out to functionally
characterize this new member of Elovl in yeast. Substrate C18:2n6, C18:3n3,
C18:3n6, C18:4n3, C20:4n6, C20:5n3, C22:4n6 and C22:5n3 were tested in this
study. Results showed that this gene has the ability to convert MUFA and C18 PUFA
into their respective products which clearly revealed that its substrate selectivity is
different from other Elovl members. Therefore, it strongly supports that the protein
could be a new member of Elovl family and hereby named as Elovl8. At the tissue
level, the Elovl8 gene expression was the highest in intestine, follow by gill, eye,
brain and liver.
Description
Keywords
Molecular cloning and functional characterization , family from a freshwater teleost