Molecular Cloning And Functional Characterization Of A Novel Elovl (Elongase) Family From A Freshwater Teleost (Oreochromis Niloticus)

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dc.contributor.authorHan, Wan Yin
dc.date.accessioned2019-09-03T01:02:00Z
dc.date.available2019-09-03T01:02:00Z
dc.date.issued2017-06
dc.description.abstractAt present, seven members of ELOVL family (ELOVL1-7) have been identified in mammals. In terms of function, they are broadly divided into those that elongate the saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA), and those that elongate the polyunsaturated fatty acids (PUFA). Recently, a group of Elovl proteins, termed as Elovl4-like, was discovered from teleost. However, these proteins have shown low identities and different in term of function with the typical identified Elovl4 protein. An interest has arisen to investigate whether this group of Elovl proteins could be a new Elovl family member or as a subfamily of the existing Elovl member. This study was undertaken to clone and analyse this Elovl gene sequence (XM_005479121.2) from a freshwater fish, Nile tilapia (Oreochromis niloticus). Results showed that the sequence of Nile tilapia Elovl elongase revealed low identities with the other identified Elovl elongase (Elovl1-7) while high identities with a group of proteins termed Elovl4-like. The results had suggested that this group of Elovl proteins can be classified as a new member of Elovl family and it is designated as putative Elovl8. An in-vitro study was carried out to functionally characterize this new member of Elovl in yeast. Substrate C18:2n6, C18:3n3, C18:3n6, C18:4n3, C20:4n6, C20:5n3, C22:4n6 and C22:5n3 were tested in this study. Results showed that this gene has the ability to convert MUFA and C18 PUFA into their respective products which clearly revealed that its substrate selectivity is different from other Elovl members. Therefore, it strongly supports that the protein could be a new member of Elovl family and hereby named as Elovl8. At the tissue level, the Elovl8 gene expression was the highest in intestine, follow by gill, eye, brain and liver.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8767
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectMolecular cloning and functional characterizationen_US
dc.subjectfamily from a freshwater teleosten_US
dc.titleMolecular Cloning And Functional Characterization Of A Novel Elovl (Elongase) Family From A Freshwater Teleost (Oreochromis Niloticus)en_US
dc.typeThesisen_US
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