Characterisation of low density lipoprotein subfraction profile and apoe genotype among diabetic patients
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Date
2003
Authors
Bebakar, Wan Mohamad Wan
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
Abstract
Diabetes mellitus is a metabolic disorder characterised by chronic hyperglycaemia and dyslipidaemia, which
places diabetics at increased risk of cardiovascular disease. We strongly believed that LDL cholesterol (LDLC),
triglycerides (TG) and apolipoprotein E (apo E) genotype are closely related in determining this high risk. This
study aims to study the effect of LDL subfraction and apo E genotype on diabetic dyslipidaemia. Normal
(n=30) and diabetes mellitus type 2 (n=35) subjects who were not on any drug treatment were studied. Lipid
biochemical analysis was performed by automated methods using commercial kits. LDL size was determined
by transmission electron microscopy. LDLI and LDL2 are large panicles while LDL3, LDL4, and LDL5 are
smaller particles and are considered detrimental to health. Apo E genotype was determined by polymerase
chain reaction and restric tion fragment length polymorphism.
Description
Keywords
diabetes