Pemencilan Dan Penyaringan Fusarium Axysporum Avirulen Dan Bakteria Endofitik Untuk Perlindungan Planlet Pisang Terhadap Penyakit Layu Fusarium
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Date
2001-03
Authors
Norsilawati, Mat Nor
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Abstract
The pathogen which cause the Fusarium wilt disease on bananas (Musa sp.)
was isolated from several sites in Balik Pulau, Pulau Pinang. The isolate was
identified as Fusarium oxysporum fsp. cubense and its virulence was confirmed.
Endophytic avirulent F oxysporum and bacteria were isolated from root tissues
or rhizosphere soil of wild banana plants from several localities in Pulau Pinang,
Kedah, Perak and Kuala Lumpur. The effectiveness of these microorganisms in
eliciting cross protection in banana plantlet ho~ts against challenge inoculati.ons by the
F oxysporum f sp. cubense pathogen were studied using the double cup pathogenicity
test technique.
Sixteen Fusarium oxysporum isolates were obtained from the roots of wild
banana plants. Fourteen isolates were found to be endophytic while three isolates
example F02, F03 and FOIl which gave the best protection against Fusarium wilt
were selected for further investigations of their effectiveness.
For the endophytic bacteria that were isolated from rhizosphere soils, 260
isolates were successfully cultured. Finally, only 14 isolates were found to produce
consistent mycelial growth inhibition and their effectiveness were studied in challenge
incubations. B7 (Bacillus sp.) was the only isolate to successfully showed disease
symptom reduction.
Test of synergistic action between avirulent fungi and endophytic bacteria gave
poorer results when compared to the use of avirulent fungi only.
Both avirulent fungi and endophytic bacteria have the potential individually to
reduce the severity of Fusarium wilt disease symptoms in banana plantlets
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Keywords
Fusarium Axysporum Avirulen , Bakteria Endofitik