Isolation And Characterisation Of Chemical Constituents With Anti-Methicilin Resistant Staphylococcus Aureus Activity From Mesua Ferrea Leaf Extract

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Date
2018-05
Authors
Alagasamy, Sangeeta Vani
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Publisher
Universiti Sains Malaysia
Abstract
The present study aims to isolate compounds with antimicrobial activity from Mesua ferrea leaf employing bioassay guided approach. An antibacterial assay guided extraction and compound isolation procedure was carried out on Mesua ferrea methanolic leaf extract with a number of Gram positives and negatives bacteria. Firstly, M. ferrea leaf extraction technique was optimized using antimicrobial assay guided method. Powdered freeze dried leaves of M. ferrea (particle size 0.5mm) (1:15w/v) using ultrasonic-assisted technique yielded methanol crude extract with a good antibacterial activity against Staphylococcus aureus, MRSA, Pseudomonas aeruginosa, Bacillus subtilis and Escherichia coli. The methanolic crude extract was further partitioned with various solvents to obtain active fractions of M. ferrea leaf. Sub-fraction (T8) demonstrated the strongest antibacterial activity against S. aureus and MRSA and was further subjected to antibacterial assay guided isolation. Four compounds were isolated of which stigmasterol and caryophyllene oxide demonstrated good antibacterial activity against S. aureus and MRSA with MIC values of 31.25, 62.5 μg/mL and 15.625, 31.25 μg/mL respectively. The MBC values of > 500 μg/mL suggested that both stigmasterol and caryophyllene oxide are bacteriostatic compounds. As for the remaining two compounds the MIC values were above 500 μg/mL. These compounds were not considered for structural elucidation.
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Keywords
Isolate compounds with antimicrobial activity , Mesua ferrea leaf employing bioassay guided approach
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