Isolation And Characterisation Of Chemical Constituents With Anti-Methicilin Resistant Staphylococcus Aureus Activity From Mesua Ferrea Leaf Extract

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dc.contributor.authorAlagasamy, Sangeeta Vani
dc.date.accessioned2019-05-13T01:00:43Z
dc.date.available2019-05-13T01:00:43Z
dc.date.issued2018-05
dc.description.abstractThe present study aims to isolate compounds with antimicrobial activity from Mesua ferrea leaf employing bioassay guided approach. An antibacterial assay guided extraction and compound isolation procedure was carried out on Mesua ferrea methanolic leaf extract with a number of Gram positives and negatives bacteria. Firstly, M. ferrea leaf extraction technique was optimized using antimicrobial assay guided method. Powdered freeze dried leaves of M. ferrea (particle size 0.5mm) (1:15w/v) using ultrasonic-assisted technique yielded methanol crude extract with a good antibacterial activity against Staphylococcus aureus, MRSA, Pseudomonas aeruginosa, Bacillus subtilis and Escherichia coli. The methanolic crude extract was further partitioned with various solvents to obtain active fractions of M. ferrea leaf. Sub-fraction (T8) demonstrated the strongest antibacterial activity against S. aureus and MRSA and was further subjected to antibacterial assay guided isolation. Four compounds were isolated of which stigmasterol and caryophyllene oxide demonstrated good antibacterial activity against S. aureus and MRSA with MIC values of 31.25, 62.5 μg/mL and 15.625, 31.25 μg/mL respectively. The MBC values of > 500 μg/mL suggested that both stigmasterol and caryophyllene oxide are bacteriostatic compounds. As for the remaining two compounds the MIC values were above 500 μg/mL. These compounds were not considered for structural elucidation.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8184
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectIsolate compounds with antimicrobial activityen_US
dc.subjectMesua ferrea leaf employing bioassay guided approachen_US
dc.titleIsolation And Characterisation Of Chemical Constituents With Anti-Methicilin Resistant Staphylococcus Aureus Activity From Mesua Ferrea Leaf Extracten_US
dc.typeThesisen_US
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