Gene Expression Of Interleukin-4 (Il-4) Cytokine In Clinacanthus Nutans - Treated Macrophages
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Date
2015
Authors
Mohamed, Siti Hajar
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Clinacanthus nutans (Burm f.) Lindau (C.nutans) is a traditionally well-known medicine in
South East Asia. It was claimed to consist several phytomedicinal properties such as antiinflammatory
activities, antioxidants and anti-herpes amongst the traditional healers.
Despite previous studies on C.nutans, many of which involve properties associated with
inhibiting the inflammatory response, understanding the mechanisms involved remain to be
elucidated. This study aims to evaluate the expression of an anti-inflammatory cytokine,
interleukin-4 (IL-4) cytokine in treated macrophages. Macrophage identification and
characterization was assessed based on the observation of morphologies under invertedphase
microscope and F4/80 expression by using flow cytometry (FACS). The cytotoxicity
of C.nutans extracts using four different polarity of solvents which are petroleum ether
(PE), chloroform (CHL), ethanol (ETOH) and aqueous (AQ) were examined using
PrestoBlue™ reagent. Percentage of reduction shows inhibition of the RAW264.7
macrophages by the PE, CHL, ETOH, and AQ-C.nutans extracts. Based on the percentage
of reduction, ETOH and AQ-C.nutans extracts show that they are less cytotoxic to the
RAW264.7 macrophages compared to the PE and CHL-C.nutans extract. In terms of IL-4
expression, with this limited findings, we concluded that there is no expression of IL-4 in
RAW264.7 cells treated with AQ-C.nutans extract. However, further studies are needed to
get complete overview of the anti-inflammatory cytokine expression in C.nutans treated
RAW264.7 macrophages.
Description
Keywords
Anti-inflammatory cytokine