Gene Expression Of Interleukin-4 (Il-4) Cytokine In Clinacanthus Nutans - Treated Macrophages

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dc.contributor.authorMohamed, Siti Hajar
dc.date.accessioned2016-09-08T05:08:52Z
dc.date.available2016-09-08T05:08:52Z
dc.date.issued2015
dc.description.abstractClinacanthus nutans (Burm f.) Lindau (C.nutans) is a traditionally well-known medicine in South East Asia. It was claimed to consist several phytomedicinal properties such as antiinflammatory activities, antioxidants and anti-herpes amongst the traditional healers. Despite previous studies on C.nutans, many of which involve properties associated with inhibiting the inflammatory response, understanding the mechanisms involved remain to be elucidated. This study aims to evaluate the expression of an anti-inflammatory cytokine, interleukin-4 (IL-4) cytokine in treated macrophages. Macrophage identification and characterization was assessed based on the observation of morphologies under invertedphase microscope and F4/80 expression by using flow cytometry (FACS). The cytotoxicity of C.nutans extracts using four different polarity of solvents which are petroleum ether (PE), chloroform (CHL), ethanol (ETOH) and aqueous (AQ) were examined using PrestoBlue™ reagent. Percentage of reduction shows inhibition of the RAW264.7 macrophages by the PE, CHL, ETOH, and AQ-C.nutans extracts. Based on the percentage of reduction, ETOH and AQ-C.nutans extracts show that they are less cytotoxic to the RAW264.7 macrophages compared to the PE and CHL-C.nutans extract. In terms of IL-4 expression, with this limited findings, we concluded that there is no expression of IL-4 in RAW264.7 cells treated with AQ-C.nutans extract. However, further studies are needed to get complete overview of the anti-inflammatory cytokine expression in C.nutans treated RAW264.7 macrophages.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/2493
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectAnti-inflammatory cytokineen_US
dc.titleGene Expression Of Interleukin-4 (Il-4) Cytokine In Clinacanthus Nutans - Treated Macrophagesen_US
dc.typeThesisen_US
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