Pengecaman dan Pengenalpastian Bakteria Fitopatogen yang Menjangkiti Kultivar Artocarpus Heterophyllus (Nangka)
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Date
2006-09
Authors
Zaidi, Nazhiatul Fateema
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Abstract
Detection and identification of phytopathogenic bacteria infecting
Artocarpus heterophyllus in this study consist of phenotype, biochemical and molecular
characteristics. Through observations, the diseased plants showed symptoms of
yellowing and wilting on leaves. In more severe cases, canker developed on larger
branch, which finally lead to the death of the whole tree. Two types of bacteria were
successfully isolated from diseased tissues taken from leaves, twigs and barks. Based on
the pathogenicity tests done on tobacco leaves and jack:fruit shoots, only one group of
bacteria were able to caused chlorosis to tobacco leaves and caused a fireburning
character to jack:fruit shoots. The other group of bacteria was later classified as
saprophyte as no positive reactions were seen on both test plants. Through morphology
test done, the first group of bacteria were gram-negative bacteria with rod-coccus shaped
cells, fimbriated and occurred in short chains where these isolates were able to ferment a
wide range of substrates, proves by the ability to grow on numerous semi selective agar.
The strains were also capable of fermenting glucose anaerobicaly. Through APIĀ® 20E
test and identification via 168 rDNA region, the strain were identified as Acinetobacter
sp. Multiple alignment of 168 rDNA region of 4 other closely related species revealed
that the strain were closely related to Acinetobacter calcoaceticus.
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Keywords
Kultivar Artocarpus Heterophyllus