Generation of reticulocytes derived from human peripheral blood CD34ᶧ haematopoietic stem/progenitor cells for plasmodium knowlesi in vitro invasion assay
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Date
2021-03
Authors
Mohamad, Fatin Sofia
Journal Title
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Publisher
Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
Abstract
presence of proerythroblasts, a large nucleated cell on day 8. The progression of
proerythroblasts into normoblast was observed on day 11 by a decrease in cell size.
Enucleated cells with at least three dots of cresyl blue ribonucleic acid (RNA) were
recognised as reticulocytes and reached its maximum at 30.00 ± 1.76% on day 14. The
invasion assay showed that P. knowlesi invaded CD34+ HSPC-derived reticulocytes,
which was confirmed by Giemsa stained observations at 24-hour post-inoculation,
however, with lower invasion index, 1.20 ± 0.33%. Meanwhile, P. falciparum
efficiently invaded CD34+ HSPC-derived reticulocytes which was observed at 41-hour
post-inoculation with an invasion index of 2.60 ± 0.11%. In conclusion, human PBderived
CD34+ HSPCs could be considered as a potential source to generate
reticulocytes required for P. knowlesi continuous in vitro culture.
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Keywords
reticulocytes