Generation of reticulocytes derived from human peripheral blood CD34ᶧ haematopoietic stem/progenitor cells for plasmodium knowlesi in vitro invasion assay

dc.contributor.author	Mohamad, Fatin Sofia
dc.date.accessioned	2021-08-22T02:24:43Z
dc.date.available	2021-08-22T02:24:43Z
dc.date.issued	2021-03
dc.description.abstract	presence of proerythroblasts, a large nucleated cell on day 8. The progression of proerythroblasts into normoblast was observed on day 11 by a decrease in cell size. Enucleated cells with at least three dots of cresyl blue ribonucleic acid (RNA) were recognised as reticulocytes and reached its maximum at 30.00 ± 1.76% on day 14. The invasion assay showed that P. knowlesi invaded CD34+ HSPC-derived reticulocytes, which was confirmed by Giemsa stained observations at 24-hour post-inoculation, however, with lower invasion index, 1.20 ± 0.33%. Meanwhile, P. falciparum efficiently invaded CD34+ HSPC-derived reticulocytes which was observed at 41-hour post-inoculation with an invasion index of 2.60 ± 0.11%. In conclusion, human PBderived CD34+ HSPCs could be considered as a potential source to generate reticulocytes required for P. knowlesi continuous in vitro culture.	en_US
dc.identifier.uri	http://hdl.handle.net/123456789/14029
dc.language.iso	en	en_US
dc.publisher	Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia	en_US
dc.subject	reticulocytes	en_US
dc.title	Generation of reticulocytes derived from human peripheral blood CD34ᶧ haematopoietic stem/progenitor cells for plasmodium knowlesi in vitro invasion assay	en_US
dc.type	Thesis	en_US

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