Antiproliferative Activity of Stingless Bee Pollen Extract on Human Breast Cancer Cell Line MCF-7
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Date
2015
Authors
Azhar, Nur Asna
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Journal ISSN
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Publisher
Universiti Sains Malaysia
Abstract
The aims of this study were to determine the antioxidant and antiproliferative activity of the bee pollen extract from stingless bee L. terminata. Antioxidant activity was determined using 2,2-diphenyl-2-picrylhydrazyl (DPPH), and Folin-Ciocalteu assays was used to test the total phenolic content in the bee pollen extract, the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) assay was used to determine antiproliferative. The effective dose (EC50) of bee pollen extract was 0.36 mg/ml. Total phenolic content of bee pollen extract at 20mg/ml of bee pollen extract was 34.93 ± 0.150mg/g.The MTT assay showed that bee pollen extract median inhibitory concentration (IC50) 25.5mg/ml for normal cell line L929 while IC50 for breast cancer cell MCF7 was 15mg/ml, this study indicates that bee pollen extracts have cytotoxic effect in cancer cells, but not in normal cells. The combination of cisplatin and bee pollen extract showed synergistic interaction in inducing the killing activity of breast cancer cell line MCF7.
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Keywords
Stingless Bee Pollen Extract