Antiproliferative Activity of Stingless Bee Pollen Extract on Human Breast Cancer Cell Line MCF-7

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dc.contributor.authorAzhar, Nur Asna
dc.date.accessioned2016-09-29T07:48:10Z
dc.date.available2016-09-29T07:48:10Z
dc.date.issued2015
dc.description.abstractThe aims of this study were to determine the antioxidant and antiproliferative activity of the bee pollen extract from stingless bee L. terminata. Antioxidant activity was determined using 2,2-diphenyl-2-picrylhydrazyl (DPPH), and Folin-Ciocalteu assays was used to test the total phenolic content in the bee pollen extract, the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) assay was used to determine antiproliferative. The effective dose (EC50) of bee pollen extract was 0.36 mg/ml. Total phenolic content of bee pollen extract at 20mg/ml of bee pollen extract was 34.93 ± 0.150mg/g.The MTT assay showed that bee pollen extract median inhibitory concentration (IC50) 25.5mg/ml for normal cell line L929 while IC50 for breast cancer cell MCF7 was 15mg/ml, this study indicates that bee pollen extracts have cytotoxic effect in cancer cells, but not in normal cells. The combination of cisplatin and bee pollen extract showed synergistic interaction in inducing the killing activity of breast cancer cell line MCF7.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/2636
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectStingless Bee Pollen Extracten_US
dc.titleAntiproliferative Activity of Stingless Bee Pollen Extract on Human Breast Cancer Cell Line MCF-7en_US
dc.typeThesisen_US
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