Molecularly Imprinted Polymer Basedmicro- Solid Phase Extraction Of Bisphenol A And Sulfonamide Antiobiotics In Aqueous Samples
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Date
2018-02
Authors
Rozaini, Muhammad Nur’ Hafiz
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
This thesis focuses on the development, validation and application of new
sample preparation methods for the determination of bisphenol A (BPA) and
sulfonamide antibiotics (SAs) in aqueous matrices. A rapid microextraction method
based on ultrasound-assisted emulsification micro-solid phase extraction
incorporated with molecularly imprinted polymer (USAE-MIP-μ-SPE) was
developed for the determination of BPA in water, beverages and the aqueous liquid
in canned foods prior to high performance liquid chromatography diode array
detector (HPLC-DAD). Under the optimized conditions, USAE-MIP-μ-SPE showed
a good linearity over a concentration range of 0.5–700 μg L-1 low limit of detection
(0.07 μg L-1), good analyte recoveries (82.2–118.9 %) and acceptable repeatability
(RSD=0.7–14.2 %, n=3) with the enrichment factor of 49. The developed method
demonstrated good selectivity and reusability for extraction of BPA and hence, the
USAE-MIP-μ-SPE is simple, rapid, inexpensive and environmentally friendly. A
facile mixed matrix membrane microextraction based on molecularly imprinted silica
gel sorbent (MISG-MMMM) was developed for the determination of three selected
sulfonamide antibiotics in water samples. The molecularly imprinted silica gel
(MISG) was prepared by a surface imprinting technique with a sol-gel process.
MISG-MMMM method demonstrated good limit of detection, in the ranges of 0.06–
0.17 μg L-1 for sulfamethoxazole, sulfadiazine and sulfamonomethoxine with a
good linearity (R2≥0.995) in the concentration of 1–500 μg L-1, good recoveries (80-
96%) and repeatability (RSD = 1.0–7.2, n=3). MISG-MMMM avoids tedious
extraction operations and requires minute amount of solvent (200 μL) and sorbent
(15 mg). MISG–MMMM is beneficial in term of simplicity, ease of operation and
delivers significant cost advantages.
Description
Keywords
Polymer based-micro-solid phase extraction of bisphenol A , sulfonamide antibiotics in aqueous samples