Mikropropagasi dan penghasilan biji benih tiruan anubia sp.
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Date
1997
Authors
Singaram, Nillammai
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Abstract
Shoot tips (0.5 - 1.0 ern) of Anubia nana were cultured in l/2MS media
containing different combinations of NAA dan BA to produce multiple shoots. Anubia
nnna in 1/2MS medium containing 4 mg/L BA without NAA produced the maximum
number of shoots per explant. Shoots produced per explant in 1 /2MS was twice more than
in MS but the shoots were smaller in size. Shoot tips cultered in solid medium produced
more multiple shoots than in liquid medium. Multiple shoot production of other Anubia
species such as Anubia congensis and Anubia lanceolata was also successfully done on
l/2MS + 3.0 mg/L BA. This showed that different species of Anubia can also be
propagated using this medium. Rooting of A nubia nana was easy in l/2MS. medium
without NAA or BA.
Shoot tips of Anubia nana obtained from the tissue culture method above
were encapsulated in alginate to produce individual beads. These beads with the explant
could be stored at 23°C for two months with a minimum of 90% viability. Amongst the
sodium alginate tested. Sodium alginate at 2.5% was found to give the best r~sults,
regenerating shoots and roots within a week. The reaction time between sodium alginate
and calcium of 30 minutes produced beads with the best texture compared to 10 and 60
.-minutes. Encapsulated shoot tips regenerated complete plantlets of normal size on l/2MS
solid medium.
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Keywords
Penghasilan biji benih , Anubia sp.