In Vitro Effect Of Malaysian Mikania Cordata Leaves Extract On Blood Coagulation Factors Level In Plasma
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Date
2016
Authors
Yusoff, Nur Yuzaiza
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Current anticoagulant agent such as warfarin and heparin have demonstrated lifethreatening
side effect and its efficacy also been doubted. So, exploration of herbal plant
been done and one of them is Mikania cordata. Mikania cordata (Bum. F.) B.L
Robinson or commonly called selaput tunggul, in Malaysia, belongs to family of
Asteraceae and it was claimed to prolonged PT (prothrombin time), APTT (activated
partial thromboplastin time) and TT (thrombin time) in early study. Thus, aim in this
study were to identify the active compound through GCMS analysis and to determine
level of factors VII, IX, X, II and FGN in plasma treated in vitro with different
concentrations of M.cordata aqueous leaves extract. As a result, in 25.0 mg/ml of M.
cordata extract, significant percentage of factor reduced can be observed which were in
FII with 90.17%, followed by FGN (89.80%), FX (80.91%), FIX (69.15%) and finally
FVII (62.67%) compared to control with p < 0.001.This finding was supported by the
identification of malic acid which was believed to possess anticoagulant activity. Malic
acid also reacts in concentration- dependent manner when 25.0 mg/ml of M. cordata
extract treated on plasma showed high reduction of factors compared to 12. 5 mg/ml. In
conclusion, it was confirmed that malic acid possess anticoagulant activity in
concentration dependent manner, by reducing level of factors VII, IX, X, FII and FGN
in plasma.
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Keywords
Mikania Cordata Leaves Extract On , Blood Coagulation Factors Level In Plasma