Effects Of Aqueous-Ethanolic Extracts And Fractions Of Moringa Oleifera Lam. Leaf On Diabetic And Metabolic Syndrome Rats
Loading...
Date
2017-08
Authors
Irfan, Hafiz Muhammad
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Recent advancements in isolation and identification of active compounds
present in medicinal plants have culminated in a fractionation-guided targeted
approach in search of bioactivities. Thus, the present research was designed to
determine the most active extract, active fraction and to identify active principle
from Moringa oleifera leaf aqueous-ethanol extracts in diabetic rats. In addition,
these extracts were also assessed in metabolic syndrome rats. Five different aqueous
ethanol extracts (95, 75, 50, 25% [v/v] ethanol and 100% water) were orally tested in
normoglycemic Sprague Dawley rats for anti-hyperglycemic activity and glucose
tolerance. Rats with streptozotocin-induced diabetes were used to assess acute and
sub-chronic anti-hyperglycaemic activities. The most active extract (95% ethanol)
was further fractionated into different polarity solvents of hexane, chloroform, ethyl
acetate, butanol and aqueous fractions and these fractions were screened for antidiabetic
activity in diabetic rats. The most active extract and fraction were then
subjected to qualitative and quantitative phytochemical analyses; thin layer
chromatography (TLC) and high performance liquid chromatography (HPLC) were
used in order to identify anti-diabetic compounds. Among all extracts and fractions,
95% ethanol extract at the dose of 1000 mg/kg and butanol fraction at the dose of
500 mg/kg were found to be most active, showing significant reduction in blood
glucose concentration upon single administration in diabetic rats. There was no
significant anti-hyperglycemic activity, and glucose tolerance was observed in
normal rats.
Description
Keywords
Effects of aqueous-ethanolic extracts and fractions , moringa oleifera lam. Leaf on diabetic