Effects Of Aqueous-Ethanolic Extracts And Fractions Of Moringa Oleifera Lam. Leaf On Diabetic And Metabolic Syndrome Rats

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dc.contributor.authorIrfan, Hafiz Muhammad
dc.date.accessioned2019-08-09T01:28:46Z
dc.date.available2019-08-09T01:28:46Z
dc.date.issued2017-08
dc.description.abstractRecent advancements in isolation and identification of active compounds present in medicinal plants have culminated in a fractionation-guided targeted approach in search of bioactivities. Thus, the present research was designed to determine the most active extract, active fraction and to identify active principle from Moringa oleifera leaf aqueous-ethanol extracts in diabetic rats. In addition, these extracts were also assessed in metabolic syndrome rats. Five different aqueous ethanol extracts (95, 75, 50, 25% [v/v] ethanol and 100% water) were orally tested in normoglycemic Sprague Dawley rats for anti-hyperglycemic activity and glucose tolerance. Rats with streptozotocin-induced diabetes were used to assess acute and sub-chronic anti-hyperglycaemic activities. The most active extract (95% ethanol) was further fractionated into different polarity solvents of hexane, chloroform, ethyl acetate, butanol and aqueous fractions and these fractions were screened for antidiabetic activity in diabetic rats. The most active extract and fraction were then subjected to qualitative and quantitative phytochemical analyses; thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) were used in order to identify anti-diabetic compounds. Among all extracts and fractions, 95% ethanol extract at the dose of 1000 mg/kg and butanol fraction at the dose of 500 mg/kg were found to be most active, showing significant reduction in blood glucose concentration upon single administration in diabetic rats. There was no significant anti-hyperglycemic activity, and glucose tolerance was observed in normal rats.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8604
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectEffects of aqueous-ethanolic extracts and fractionsen_US
dc.subjectmoringa oleifera lam. Leaf on diabeticen_US
dc.titleEffects Of Aqueous-Ethanolic Extracts And Fractions Of Moringa Oleifera Lam. Leaf On Diabetic And Metabolic Syndrome Ratsen_US
dc.typeThesisen_US
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