Expression Of Monoclonal Antibody IBMR3 Antigen In Breast Cancer Tissue
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Date
2016-09
Authors
Gazem, Ekhlas Qaid
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
The detection of several types of cancer is possible by using monoclonal
antibody (MAb). Studies have shown that different types of cancer have related
specific abnormal proteins, which are identified by MAb. IBMR3 is a MAb which
was raised against synthetic peptides that are associated with the selected amino acid
sequence of human interleukin-4 receptors. This research is to detect the expression
of IBMR3 antigen in carcinoma including Human laryngeal epithelial carcinoma
(HEp-2), Human colon adenocarcinoma (HT-29) and breast cancer tissues. The
expression of IBMR3 antigen was detected on HEp-2 and HT-29 using indirect
immunofluorescent staining, flow cytometry staining. The results showed the
possible association of IBMR3 antigen expression in carcinoma (epithelial cancer
cells) with positive cytoplasmic staining. HEp-2 and HT-29 were separated via 1-D
immunoblot and the results showed 2 bands with molecular weights (MWs) of 45
and 25 kDa in HT-29 and one band with MWs of 25 kDa in HEp-2. IBMR3 antigen
expression was detected on 48 samples of Malaysian breast cancer and microarray
(TMA) slides of 430 breast cancer tissues and 132 normal breast tissues adjacent to
breast cancer through IHC. The expression of this antigen significantly correlated to
the histological grades, tumour stages, and lymph node metastases. High IBMR3
expression was observed in grade I cancerous breast tissues compared with the
expression observed grade III in both Malaysian breast cancer samples (P < 0.003)
and TMA slides (P < 0.01). Human breast carcinoma tissues were separated via 1-D
immunoblot.
Description
Keywords
Monoclonal antibody IBMR3 antigen , in breast cancer tissue