Expression Of Monoclonal Antibody IBMR3 Antigen In Breast Cancer Tissue

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dc.contributor.authorGazem, Ekhlas Qaid
dc.date.accessioned2019-07-12T07:31:06Z
dc.date.available2019-07-12T07:31:06Z
dc.date.issued2016-09
dc.description.abstractThe detection of several types of cancer is possible by using monoclonal antibody (MAb). Studies have shown that different types of cancer have related specific abnormal proteins, which are identified by MAb. IBMR3 is a MAb which was raised against synthetic peptides that are associated with the selected amino acid sequence of human interleukin-4 receptors. This research is to detect the expression of IBMR3 antigen in carcinoma including Human laryngeal epithelial carcinoma (HEp-2), Human colon adenocarcinoma (HT-29) and breast cancer tissues. The expression of IBMR3 antigen was detected on HEp-2 and HT-29 using indirect immunofluorescent staining, flow cytometry staining. The results showed the possible association of IBMR3 antigen expression in carcinoma (epithelial cancer cells) with positive cytoplasmic staining. HEp-2 and HT-29 were separated via 1-D immunoblot and the results showed 2 bands with molecular weights (MWs) of 45 and 25 kDa in HT-29 and one band with MWs of 25 kDa in HEp-2. IBMR3 antigen expression was detected on 48 samples of Malaysian breast cancer and microarray (TMA) slides of 430 breast cancer tissues and 132 normal breast tissues adjacent to breast cancer through IHC. The expression of this antigen significantly correlated to the histological grades, tumour stages, and lymph node metastases. High IBMR3 expression was observed in grade I cancerous breast tissues compared with the expression observed grade III in both Malaysian breast cancer samples (P < 0.003) and TMA slides (P < 0.01). Human breast carcinoma tissues were separated via 1-D immunoblot.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8473
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectMonoclonal antibody IBMR3 antigenen_US
dc.subjectin breast cancer tissueen_US
dc.titleExpression Of Monoclonal Antibody IBMR3 Antigen In Breast Cancer Tissueen_US
dc.typeThesisen_US
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