Synthesis And Characterisation Of Silver Nanoparticles Using Vernonia cinerea Aqueous Extract And Their Cytotoxic Activity Against Kasumi-1 Cell Line
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Date
2016-01
Authors
Mohamad Zani, Radhiyatul Akma
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Nanotechnology has gained attention due to their wide applications in various areas
such as cancer diagnosis and therapy. Production of silver nanoparticles (AgNPs)
using plant extracts is safer and cost-effective compared to chemical and physical
methods. In this study, AgNPs were produced using 1:5, 1:10 and 1:20 ratios of
Vernonia cinerea (V. cinerea) aqueous extract and silver nitrate solution. The
mixtures were incubated for 24 hour at 40 ˚C water baths. The formation of silver
nanoparticles was monitored for colour changes and characterized by UV-Vis
spectroscopy followed by transmission electron microscopy (TEM) and zeta
potential analyses. The UV-Vis spectrum of AgNPs showed absorption maxima at
450 nm while TEM images showed that the shape of AgNPs is spherical in shape
with an average size of 15.29 nm. The zeta value of synthesised V. cinerea-AgNPs is
– 29.50 mV with a peak area of 100 % intensity. This indicates that the V. cinerea-
AgNPs are fairly stable. The cytotoxicity activity was tested in vitro against Kasumi-
1 cell line where the proliferative activity of V. cinerea-AgNPs was measured using
MTS assay. The IC50 values of V. cinerea-AgNPs on Kasumi-1 cells were 24.42
μg/mL, 25.39 μg/mL and 21.49 μg/mL at 24, 48 and 72 hours of incubations. On the
other hand, the IC50 values of V. cinerea aqueous extract on Kasumi-1 cells were
210.53 μg/mL and 131.58 μg/mL at 48 and 72 hours respectively but no significant
difference on the Kasumi-1 cells at 24 hours incubation. Therefore, it is concluded
that V. cinerea extract has potential in producing AgNPs extracellularly and used as
anticancer agents particularly on Kasumi-1 cells.
Description
Keywords
Synthesis and characterisation of silver nanoparticles , cytotoxic activity against kasumi-1 cell lines