Synthesis And Characterisation Of Silver Nanoparticles Using Vernonia cinerea Aqueous Extract And Their Cytotoxic Activity Against Kasumi-1 Cell Line

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dc.contributor.authorMohamad Zani, Radhiyatul Akma
dc.date.accessioned2019-07-16T08:04:48Z
dc.date.available2019-07-16T08:04:48Z
dc.date.issued2016-01
dc.description.abstractNanotechnology has gained attention due to their wide applications in various areas such as cancer diagnosis and therapy. Production of silver nanoparticles (AgNPs) using plant extracts is safer and cost-effective compared to chemical and physical methods. In this study, AgNPs were produced using 1:5, 1:10 and 1:20 ratios of Vernonia cinerea (V. cinerea) aqueous extract and silver nitrate solution. The mixtures were incubated for 24 hour at 40 ˚C water baths. The formation of silver nanoparticles was monitored for colour changes and characterized by UV-Vis spectroscopy followed by transmission electron microscopy (TEM) and zeta potential analyses. The UV-Vis spectrum of AgNPs showed absorption maxima at 450 nm while TEM images showed that the shape of AgNPs is spherical in shape with an average size of 15.29 nm. The zeta value of synthesised V. cinerea-AgNPs is – 29.50 mV with a peak area of 100 % intensity. This indicates that the V. cinerea- AgNPs are fairly stable. The cytotoxicity activity was tested in vitro against Kasumi- 1 cell line where the proliferative activity of V. cinerea-AgNPs was measured using MTS assay. The IC50 values of V. cinerea-AgNPs on Kasumi-1 cells were 24.42 μg/mL, 25.39 μg/mL and 21.49 μg/mL at 24, 48 and 72 hours of incubations. On the other hand, the IC50 values of V. cinerea aqueous extract on Kasumi-1 cells were 210.53 μg/mL and 131.58 μg/mL at 48 and 72 hours respectively but no significant difference on the Kasumi-1 cells at 24 hours incubation. Therefore, it is concluded that V. cinerea extract has potential in producing AgNPs extracellularly and used as anticancer agents particularly on Kasumi-1 cells.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8503
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectSynthesis and characterisation of silver nanoparticlesen_US
dc.subjectcytotoxic activity against kasumi-1 cell linesen_US
dc.titleSynthesis And Characterisation Of Silver Nanoparticles Using Vernonia cinerea Aqueous Extract And Their Cytotoxic Activity Against Kasumi-1 Cell Lineen_US
dc.typeThesisen_US
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