The in-vitro effects of 'gamat' extract of stichopus species on human osteoblast cell line
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Date
2006-11
Authors
Ahmad, Shahrulazua
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
Abstract
Abstract
"Gamat", a local term for sea cucumber, is widely used and had been shown in
various studies to have many therapeutic effects. However, its action on osteoblast cells had
never been investigated before. Hence, in-vitro study utilising a commercially produced
gamat extract of Stichopus sp 1 as the test substance was performed to elucidate the effects
on the osteoblast cell line proliferation and functional activity, using MTT colorimetric
assay and alkaline phosphatase (ALP) assay respectively.
In the preliminary study, osteoblast cells were mixed with a series of two-fold
dilutions of gamat concentration in standard culture media from lOOmg/ml down to
1.6mg/ml, and with negative as well as positive controls. Results that were noted after 72
hours incubation period showed an inverse relationship between the gamat concentration
and its effect on the osteoblast cell proliferation. The exact ICso was estimated to be
approximately 75mg/ml. There was a positive promoting effect of gamat extract on
osteoblast cell functional activity when 1.6mg/ml, 3.1mg/ml, 6.2mg/ml, 12.5mg/ml, and
25mg/ml of gamat concentrations were used. Although gamat at 1 OOmg/ml showed the
Description
Keywords
Osteoblast