The in-vitro effects of 'gamat' extract of stichopus species on human osteoblast cell line
| dc.contributor.author | Ahmad, Shahrulazua | |
| dc.date.accessioned | 2020-10-25T01:37:18Z | |
| dc.date.available | 2020-10-25T01:37:18Z | |
| dc.date.issued | 2006-11 | |
| dc.description.abstract | Abstract "Gamat", a local term for sea cucumber, is widely used and had been shown in various studies to have many therapeutic effects. However, its action on osteoblast cells had never been investigated before. Hence, in-vitro study utilising a commercially produced gamat extract of Stichopus sp 1 as the test substance was performed to elucidate the effects on the osteoblast cell line proliferation and functional activity, using MTT colorimetric assay and alkaline phosphatase (ALP) assay respectively. In the preliminary study, osteoblast cells were mixed with a series of two-fold dilutions of gamat concentration in standard culture media from lOOmg/ml down to 1.6mg/ml, and with negative as well as positive controls. Results that were noted after 72 hours incubation period showed an inverse relationship between the gamat concentration and its effect on the osteoblast cell proliferation. The exact ICso was estimated to be approximately 75mg/ml. There was a positive promoting effect of gamat extract on osteoblast cell functional activity when 1.6mg/ml, 3.1mg/ml, 6.2mg/ml, 12.5mg/ml, and 25mg/ml of gamat concentrations were used. Although gamat at 1 OOmg/ml showed the | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/10586 | |
| dc.language.iso | en | en_US |
| dc.publisher | Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia | en_US |
| dc.subject | Osteoblast | en_US |
| dc.title | The in-vitro effects of 'gamat' extract of stichopus species on human osteoblast cell line | en_US |
| dc.type | Thesis | en_US |
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