Small Rna (Srna) Regulates Extreme Temperature Adaptation Of Geobacillus Thermoleovorans Isolated From A Malaysian Hot Spring
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Date
2011-02
Authors
Tan, Hock Siew
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Small RNA (sRNA) has been shown to play important gene regulatory roles
in both eukaryotes (commonly known as microRNA) and prokaryotes. In bacteria,
sRNAs regulate biofilm formation, quorum sensing, virulence, and environmental
stress response. Of the various stress responses, the heat shock response has been
extensively studied in many pathogenic bacteria and extremophiles. The focus of this
study is to identify the sRNAs that may be involved in the adaptation to 60°C and
70°C of a thermophilic bacterium, Geobacillus thermoleovorans CCB_US3_UF5.
The sRNAs from these extreme high temperature growths were reverse transcribed to
cDNA and sequenced. Sequencing data identified 83 putative sRNAs classified as
antisense, intergenic region, untranslated region, or non-coding. Out of this total, 44
sRNA candidates were specific to growth at elevated temperature. This study
demonstrated a complete sRNA analysis workflow for a thermophilic bacteria by
combining a sRNA cloning protocol and high-throughput sequencing data analysis
pipeline. Collectively, these results can be used for future studies to better understand
the detailed sRNA regulation of extreme temperature adaptation in thermophilic
bacteria.
Description
Keywords
Small Rna (Srna) , Geobacillus Thermoleovorans