Small Rna (Srna) Regulates Extreme Temperature Adaptation Of Geobacillus Thermoleovorans Isolated From A Malaysian Hot Spring

Simple item page
dc.contributor.authorTan, Hock Siew
dc.date.accessioned2019-10-08T03:15:54Z
dc.date.available2019-10-08T03:15:54Z
dc.date.issued2011-02
dc.description.abstractSmall RNA (sRNA) has been shown to play important gene regulatory roles in both eukaryotes (commonly known as microRNA) and prokaryotes. In bacteria, sRNAs regulate biofilm formation, quorum sensing, virulence, and environmental stress response. Of the various stress responses, the heat shock response has been extensively studied in many pathogenic bacteria and extremophiles. The focus of this study is to identify the sRNAs that may be involved in the adaptation to 60°C and 70°C of a thermophilic bacterium, Geobacillus thermoleovorans CCB_US3_UF5. The sRNAs from these extreme high temperature growths were reverse transcribed to cDNA and sequenced. Sequencing data identified 83 putative sRNAs classified as antisense, intergenic region, untranslated region, or non-coding. Out of this total, 44 sRNA candidates were specific to growth at elevated temperature. This study demonstrated a complete sRNA analysis workflow for a thermophilic bacteria by combining a sRNA cloning protocol and high-throughput sequencing data analysis pipeline. Collectively, these results can be used for future studies to better understand the detailed sRNA regulation of extreme temperature adaptation in thermophilic bacteria.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8970
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectSmall Rna (Srna)en_US
dc.subjectGeobacillus Thermoleovoransen_US
dc.titleSmall Rna (Srna) Regulates Extreme Temperature Adaptation Of Geobacillus Thermoleovorans Isolated From A Malaysian Hot Springen_US
dc.typeThesisen_US
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Pusat Pengajian Sains Kajihayat - Tesis