Antihyperglycemic Activity Of Tinospora Crispa Extracts And Fractions In Rat

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dc.contributor.authorSEYED HASSANI, MIR MOHAMMAD REZA
dc.date.accessioned2016-05-25T07:07:24Z
dc.date.available2016-05-25T07:07:24Z
dc.date.issued2015-08
dc.description.abstractTinospora crispa (Menispermaceae), locally known as “akar patawali”, has long been used in Malaysian traditional medicine for treatment of diabetes. The aim of this study was to verify the hypoglycemic and antihyperglycemic effects of different Tinospora crispa extracts in normal and streptozotocin (STZ)-induced diabetic rats. Pulverized Tinospora crispa dried stems were extracted successively by maceration with petroleum ether, chloroform, methanol and water to yield the four extracts. None of the four extracts (1.0 g/kg) administered orally lowered the blood glucose levels of normal rats which suggest that they have no hypoglycemic effect. The four extracts also did not inhibit the rise of blood glucose level of glucose intraperitoneally loaded rats. Daily oral administration of the extract 1 g/kg for 9 days in STZ-induced diabetic rats found that only the water extract managed to lower the blood glucose levels significantly (P<0.05), as compared with the control group which suggests that it has antihyperglycaemic effect. The water extract was then fractionated to obtain n-butanol and aqueous fractions. However, daily treatment for 12 days found that only water fraction (0.5 g/kg) lowered the blood glucose level of STZ-induced diabetic rats. Phytochemical screening of the water extracts of Tinospora crispa indicated the presence of alkaloids, glycosides and saponins. GC-MS of water fraction showed the presence of dodecyl acrylate, pentadecanoic acid and propanoic acid whereas HPLC analysis showed the presence of gallic acid and salsolinol. Dodecyl acrylate, pentadecanoic acid, propanoic acid and gallic acid have been previously reported to possess antidiabetic effect. Overall, xviii the findings of this study showed Tinospora crispa possesses antidiabetic effect which may justified its use in diabetic patients. Some of the identified compounds above might have contributed individually or in combination to its antidiabetic activity.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/2050
dc.subjectAntihyperglycemic Activity Of Tinospora Crispaen_US
dc.subjectExtracts And Fractions In Raten_US
dc.titleAntihyperglycemic Activity Of Tinospora Crispa Extracts And Fractions In Raten_US
dc.typeThesisen_US
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