Screening And Characterisation Of Inhibitors For Glyoxylate Cycle ICL Enzyme Activity In Candida Albicans
Loading...
Date
2014-06
Authors
Cheah, Hong Leong
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Candida albicans is an opportunistic pathogen that causes candidiasis in human. Metabolic pathways have been recently explored as potential antimicrobial targets, for example glyoxylate cycle that enables the C. albicans to servive nutrient-limited niches. Glyoxylate cycle and its key enzymes would be an attractive antifungal drug target for C. albicans. In this study, an alternative cell-based screening approach that better reflect the physiological environment in host system was employed to identify lead compounds that inhibit the glyoxylate cycle. The lead compounds identified are caffeic acid, rosmarinic acid and apigenin, which were previously undervalued, but demostrated to have antifungal activity against C. albicans when assayed under physiologically relevant conditions, and have inhibitory property on isocitrate lyase (ICL) enzyme activity.
Description
Keywords
Candida albicans is an opportunistic pathogen , hat causes candidiasis in human