Genotoxic evaluation of locally produced dental porcelain using the Ames salmonella and comet assays
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Date
2009
Authors
Mohammed Noushad
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Abstract
Porcelain is the most natural-appearing synthetic replacement dental restorative
material, holding a special place in dentistry because of its most aesthetically pleasing
result. Even though dental porcelains are generally considered to be inert, their
biocompatibility cannot be overlooked as these restorations stay in the oral cavity for
years or even decades. The aim of this study was to determine the genotoxicity of locally
produced dental porcelain (Universiti Sains Malaysia, Malaysia) using the
Salmonella/mammalian-microsome mutagenicity assay (Ames assay) and the single cell
gel electrophoresis assay (Comet assay). In the Ames assay, four genotypic variants of
the Salmonella strains (TA98, TA100, TA1535 and TA1537) carrying mutations in
several genes were used. The dental porcelain was incubated with these four strains at
five different concentrations (0.3125, 0.625, 1.25, 2.5 and 5 mg/plate) along with
concurrent appropriate positive and negative controls both in the absence and presence
of metabolic activation (S9). The results were assessed based on the number of revertant
colonies/plate and if it was more than double the number than that of the negative
control, the results are considered mutagenic. In the Comet assay, L929 (CCL-1 ATCC,
USA) mouse fibroblast cells were treated with the locally produced dental porcelain at
three different concentrations (50, 100 and 200 mg/ml) along with concurrent negative
and positive controls. The results of the Comet assay were assessed based on the tail
moment, which was used as the parameter to determine the DNA damage and compared
to that of the negative control. In the Ames assay, the average number of revertant
colonies per plate treated with locally produced dental porcelain was less than double as
compared to that of the negative control, whereas in the case of Comet assay, the tail
moment was almost equal to that of the negative control. From the results of the current
study, it is inferred that the locally produced dental porcelain is non-genotoxic under the
present test conditions.
Description
Master
Keywords
Dentistry , Genotoxic , Dental porcelain , Ames salmonella , Comet assays