Genotoxic evaluation of locally produced dental porcelain using the Ames salmonella and comet assays

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dc.contributor.authorMohammed Noushad
dc.date.accessioned2014-11-03T02:14:58Z
dc.date.available2014-11-03T02:14:58Z
dc.date.issued2009
dc.descriptionMasteren_US
dc.description.abstractPorcelain is the most natural-appearing synthetic replacement dental restorative material, holding a special place in dentistry because of its most aesthetically pleasing result. Even though dental porcelains are generally considered to be inert, their biocompatibility cannot be overlooked as these restorations stay in the oral cavity for years or even decades. The aim of this study was to determine the genotoxicity of locally produced dental porcelain (Universiti Sains Malaysia, Malaysia) using the Salmonella/mammalian-microsome mutagenicity assay (Ames assay) and the single cell gel electrophoresis assay (Comet assay). In the Ames assay, four genotypic variants of the Salmonella strains (TA98, TA100, TA1535 and TA1537) carrying mutations in several genes were used. The dental porcelain was incubated with these four strains at five different concentrations (0.3125, 0.625, 1.25, 2.5 and 5 mg/plate) along with concurrent appropriate positive and negative controls both in the absence and presence of metabolic activation (S9). The results were assessed based on the number of revertant colonies/plate and if it was more than double the number than that of the negative control, the results are considered mutagenic. In the Comet assay, L929 (CCL-1 ATCC, USA) mouse fibroblast cells were treated with the locally produced dental porcelain at three different concentrations (50, 100 and 200 mg/ml) along with concurrent negative and positive controls. The results of the Comet assay were assessed based on the tail moment, which was used as the parameter to determine the DNA damage and compared to that of the negative control. In the Ames assay, the average number of revertant colonies per plate treated with locally produced dental porcelain was less than double as compared to that of the negative control, whereas in the case of Comet assay, the tail moment was almost equal to that of the negative control. From the results of the current study, it is inferred that the locally produced dental porcelain is non-genotoxic under the present test conditions.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/259
dc.language.isoenen_US
dc.subjectDentistryen_US
dc.subjectGenotoxicen_US
dc.subjectDental porcelainen_US
dc.subjectAmes salmonellaen_US
dc.subjectComet assaysen_US
dc.titleGenotoxic evaluation of locally produced dental porcelain using the Ames salmonella and comet assaysen_US
dc.typeThesisen_US
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