Identification Of Biomarkers For Colorectal Cancer From Malaysian Patients
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Date
2010-06
Authors
Yeoh, Lay Chin
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Abstract
Colorectal cancer (CRC) is on the rise. However, biomarkers for
indication of CRC progression is still lacking despite several advances in
proteomic and microarray tools. This study aims to identify potential biomarkers
for the diagnosis of CRC among Malaysian patients. In this study, sequential
extraction was first performed using Tris buffer followed by TLB buffer in order
to reduce the complexity of the CRC proteome. The tissues used were surgical
resections of normal mucosa and cancerous tissues from 26 CRC patients. The
extracted proteins were separated by 2D gel electrophoresis. The differentially
expressed protein spots were then mapped for further protein identification by the
use of liquid chromatography tandem mass spectrometry (LC-MS/MS). We have
identified 37 hydrophilic proteins in the Tris extracts and 24 hydrophobic
proteins in the thiourea lysis buffer (TLB) extracts from the samples collected.
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Keywords
Rectum , Cancer