Anticancer Activity Of Silver Nanoparticles Synthesised By Catharanthus Roseus Aqueous Extract
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Date
2015
Authors
Noordin, Nor Jalilahtul Mahfuzah
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Silver nanoparticles (AgNPs) are increasingly used in biomedical applications because of
their remarkable antimicrobial activity. However, the anticancer activity of AgNPs remain
to be investigated. The present study was aimed to evaluate in vitro anticancer activity of
AgNPs on Jurkat (leukemic T-cells) and HT29 (colorectal cancer) cancer cell lines. The
biogenic AgNPs were synthesised using Catharanthus roseus(C. roseus) leaf aqueous
extract. The proliferative activity of C. roseus-AgNPs was measured using MTS assay,
followed by the detection of apoptosis using annexin-FITC/propidium iodide assay and cell
cycle activity using cell cycle assay. The IC50 values of C. roseus-AgNPs on Jurkat cells
were in the range of 1.96 to 6.26 μg/mL (median = 5.87 μg/mL) at various incubation
times. Meanwhile, the IC50 values on HT29 cells were in the range of 12.99 to 13.39 μg/mL
(median = 13.19 μg/mL) at various incubation times. In comparison, the IC50 values of C.
roseus aqueous extract on Jurkat cells were in the range of 23.43 to 700.00 μg/mL (median
= 361.72 μg/mL) while the IC50 values on HT29 cells were in the range of 191.47 to 888.00
μg/mL (median = 419.26 μg/mL) at various incubation times. Higher percentages of both
early and late apoptotic cells were detected with for Jurkat cells compared to HT29 cells at
various incubation times, in response to either C. roseus-AgNPs or C. roseus aqueous
extract. C. roseus-AgNPs and C. roseus aqueous extract have arrested S phase in Jurkat
cells at all incubations while in HT29, the C. roseus-AgNPs and C. roseus aqueous extract
have arrested either G0/G1 and S phase at various incubations. The findings indicate that
the C. roseus-AgNPs have a potential to be used as anticancer agents, particularly on
leukemia and colon cancer.
Description
Keywords
Anticancer activity