Anticancer Activity Of Silver Nanoparticles Synthesised By Catharanthus Roseus Aqueous Extract
| dc.contributor.author | Noordin, Nor Jalilahtul Mahfuzah | |
| dc.date.accessioned | 2016-09-07T07:58:20Z | |
| dc.date.available | 2016-09-07T07:58:20Z | |
| dc.date.issued | 2015 | |
| dc.description.abstract | Silver nanoparticles (AgNPs) are increasingly used in biomedical applications because of their remarkable antimicrobial activity. However, the anticancer activity of AgNPs remain to be investigated. The present study was aimed to evaluate in vitro anticancer activity of AgNPs on Jurkat (leukemic T-cells) and HT29 (colorectal cancer) cancer cell lines. The biogenic AgNPs were synthesised using Catharanthus roseus(C. roseus) leaf aqueous extract. The proliferative activity of C. roseus-AgNPs was measured using MTS assay, followed by the detection of apoptosis using annexin-FITC/propidium iodide assay and cell cycle activity using cell cycle assay. The IC50 values of C. roseus-AgNPs on Jurkat cells were in the range of 1.96 to 6.26 μg/mL (median = 5.87 μg/mL) at various incubation times. Meanwhile, the IC50 values on HT29 cells were in the range of 12.99 to 13.39 μg/mL (median = 13.19 μg/mL) at various incubation times. In comparison, the IC50 values of C. roseus aqueous extract on Jurkat cells were in the range of 23.43 to 700.00 μg/mL (median = 361.72 μg/mL) while the IC50 values on HT29 cells were in the range of 191.47 to 888.00 μg/mL (median = 419.26 μg/mL) at various incubation times. Higher percentages of both early and late apoptotic cells were detected with for Jurkat cells compared to HT29 cells at various incubation times, in response to either C. roseus-AgNPs or C. roseus aqueous extract. C. roseus-AgNPs and C. roseus aqueous extract have arrested S phase in Jurkat cells at all incubations while in HT29, the C. roseus-AgNPs and C. roseus aqueous extract have arrested either G0/G1 and S phase at various incubations. The findings indicate that the C. roseus-AgNPs have a potential to be used as anticancer agents, particularly on leukemia and colon cancer. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/2480 | |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Sains Malaysia | en_US |
| dc.subject | Anticancer activity | en_US |
| dc.title | Anticancer Activity Of Silver Nanoparticles Synthesised By Catharanthus Roseus Aqueous Extract | en_US |
| dc.type | Thesis | en_US |
Files
License bundle
1 - 1 of 1
Loading...
- Name:
- license.txt
- Size:
- 1.71 KB
- Format:
- Item-specific license agreed upon to submission
- Description: