Production Of Mannanase By Aspergillus Niger Usm F4 Via Solid Substrate Fermentation Utilizing Locust Bean Gum And Rice Husk
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Date
2011-08
Authors
Puspitaloka, Hapsari
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
The abundant of agricultural wastes can cause serious environmental
problems such as air-pollution, which is resulted from the wastes burning activity
that usually done by farmers after the harvesting season. In order to overcome the
environmental problems, the present study was undertaken by using the agricultural
wastes as the co-substrates in the SSF system for mannanase production by
Aspergillus niger USM F4. The study was focused on the physical and chemical
improvements of mannanase in a flask and tray systems, the mannanase purification
and the characterization of the purified mannanase. Compared to the other cosubstrates
(oil palm frond, rice straw and sugar cane baggase), rice husk was found
as the preferable co-substrate for mannanase production. The improvement of
mannanase in a flask system was carried out and the improved conditions were 5 g of
solid substrate using 0.75 mm particle size of co-substrate, with ratio moisture
content of 1:1 (w:v), cultivation temperature of 30°C, inoculum size of 6x106
spores/ml, static condition, with the additional of 1.6% (w/w) LBG (which act as
additional carbon source and main substrate for A. niger USM F4 to produce
mannanase) and 1.2% (w/w) ammonium nitrate. Under improved physical and
chemical conditions in a flask system, the yield of mannanase achieved up to 68.53
U/g substrate. There was an increment of 4078.66% compared to the activity before
improvement.
Description
Keywords
Mannanase By Aspergillus Niger Usm F4 , Locust Bean Gum And Rice Husk