Isolation, Genome Sequencing, Assembly And Annotation Of Thermophilic Geobacillus Thermoleovorans Ccb_Us3_Uf5 From Ulu Slim Hot Spring, Perak, Malaysia
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Date
2011-02
Authors
Muhd Sakaff, Muhd Khairul Luqman
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
A thermophilic bacterium Geobacillus thermoleovorans CCB_US3_UF5 was
isolated from Ulu Slim hot spring located in Perak, Malaysia. Understanding the
survival of G. thermoleovorans CCB_US3_UF5 requires knowledge of its genome as
the blueprint for high temperature adaptation to the environment. Genome
sequencing of the bacterium was performed using next generation sequencing
technology. The newly isolated G. thermoleovorans CCB_US3_UF5 is closely
related to thermophilic bacterium G. kaustophilus HTA 426. The draft genome
consists of 7 contigs totaling 3,506,524 bp with a mean GC content of 52.3%. The
annotation predicts 3955 genes, 73 tRNA, 23 rRNA (7 rRNA operon), 37 phage
related genes, and 117 transposase genes. Genome-based metabolic reconstruction
indicates that G. thermoleovorans CCB_US3_UF5 has a functional glycolysis, citric
acid cycle, pentose phosphate and fatty acid biosynthesis pathway.
Description
Keywords
Thermophilic Geobacillus Thermoleovorans , Ccb_Us3_Uf5