Isolation And Evaluation Of cDNA Clones Specific To Polyclonal Antibody Against 12 kDa Excretory Secretory Antigen (ESA) Of Taxoplasma Gondii
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Date
2012-06
Authors
Abdul Aziz, Farhanah
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Toxoplasmosis is a zoonotic infection caused by Toxoplasma gondii. It is an obligate
intracellular protozoan parasite distributed worldwide, with higher prevalence in the
tropical countries. Although the risk of an overt disease in immunocompetent
individuals is not high, severe manifestation may result when immunocompromised
individuals and pregnant women are infected. It is very challenging yet crucial to
establish whether acute infection has occurred in pregnant women as the fetus could be
at risk of infection which can cause death or permanent organ damage. The diagnostic
potential of various antigens have been studied to address this issue as a good
diagnostic tool is essential to determine the acute stage of the infection. The active
secretion of antigens by T. gondii i.e. excreted-secreted antigens (ESA) has been found
to stimulate a better immune response than the parasite soluble antigen. Thus, ESA is a
potential antigen for research.
Previously 12 kDa band from T. gondii ESA has been determined as an acute infection
marker with good diagnostic potential. In this study, ESA was produced from
tachyzoites harvested from mice peritoneal exudates and subjected to SDS-PAGE. The
12 kDa protein band was excised and used to produce polyclonal antibody in rabbits.
Two types of immunization strategies were employed in this study namely
immunization using protein from acrylamide gel pieces, and immunization using
electro-eluted protein mixed with adjuvant.
Description
Keywords
Isolation and evaluation of cDNA clones specific , to polyclonal antibody against 12 kDa