Isolation And Evaluation Of cDNA Clones Specific To Polyclonal Antibody Against 12 kDa Excretory Secretory Antigen (ESA) Of Taxoplasma Gondii
| dc.contributor.author | Abdul Aziz, Farhanah | |
| dc.date.accessioned | 2019-06-19T01:32:36Z | |
| dc.date.available | 2019-06-19T01:32:36Z | |
| dc.date.issued | 2012-06 | |
| dc.description.abstract | Toxoplasmosis is a zoonotic infection caused by Toxoplasma gondii. It is an obligate intracellular protozoan parasite distributed worldwide, with higher prevalence in the tropical countries. Although the risk of an overt disease in immunocompetent individuals is not high, severe manifestation may result when immunocompromised individuals and pregnant women are infected. It is very challenging yet crucial to establish whether acute infection has occurred in pregnant women as the fetus could be at risk of infection which can cause death or permanent organ damage. The diagnostic potential of various antigens have been studied to address this issue as a good diagnostic tool is essential to determine the acute stage of the infection. The active secretion of antigens by T. gondii i.e. excreted-secreted antigens (ESA) has been found to stimulate a better immune response than the parasite soluble antigen. Thus, ESA is a potential antigen for research. Previously 12 kDa band from T. gondii ESA has been determined as an acute infection marker with good diagnostic potential. In this study, ESA was produced from tachyzoites harvested from mice peritoneal exudates and subjected to SDS-PAGE. The 12 kDa protein band was excised and used to produce polyclonal antibody in rabbits. Two types of immunization strategies were employed in this study namely immunization using protein from acrylamide gel pieces, and immunization using electro-eluted protein mixed with adjuvant. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/8326 | |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Sains Malaysia | en_US |
| dc.subject | Isolation and evaluation of cDNA clones specific | en_US |
| dc.subject | to polyclonal antibody against 12 kDa | en_US |
| dc.title | Isolation And Evaluation Of cDNA Clones Specific To Polyclonal Antibody Against 12 kDa Excretory Secretory Antigen (ESA) Of Taxoplasma Gondii | en_US |
| dc.type | Thesis | en_US |
Files
License bundle
1 - 1 of 1
Loading...
- Name:
- license.txt
- Size:
- 1.71 KB
- Format:
- Item-specific license agreed upon to submission
- Description: