THE EFFECT OF DIETARY CRUDE PALM OIL AND ALPHA-TOCOPHERYL ACETATE ON REPRODUCTIVE PERFORMANCE AND LARVAL QUALITY OF TILAPIA (Oreochromis sp.)
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Date
2014-09
Authors
YAN, WANG
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Abstract
The present dissertation was conducted to investigate effects of the broodstock
dietary lipids and vitamin E on the reproductive performance of tilapia and the quality
of larvae hatched. Four isonitrogenous (35% protein) and isolipidic (10%) caseinbased
diets were formulated with added fish oil (FO), FO and crude palm oil (FO+CPO;
1:1), CPO or linseed oil as the lipid source in Experiment 1, and supplemented with 0
(T0), 60 (T60), 600 (T600) mg α-tocopheryal acetate, or a CPO-derived tocotrienolsrich
fraction designated to provide α-tocopherol at a level of 60 (PalmE60) mg per kg
dry diet in Experiment 2. Pre-spawning female tilapia (Oreochromis sp.) was
individually color-tagged, and six females and two males were stocked into a one-ton
breeding tank. Each diet was fed to two tanks of broodfish and the reproductive
performance of 12 individual female fish was monitored over 25 and 12 weeks,
respectively. Female broodfish fed the two crude palm oil-based diets and the 600 mg
α-tocopheryal acetate/kg added diet, showed significantly (P<0.05) larger ovary sizes,
had the highest number of actively spawning tilapia and produced the highest total
number of eggs per fish due to the shorter inter spawning interval and higher spawning
frequency. The hatchability of eggs originated from these dietary treatments was also
significantly higher. The inclusion of various dietary lipid source significantly affected
the fatty acid composition or vitamin E concentration of muscle, liver, ovary, testes,
egg and newly hatched larvae. In Expeirment 3, yolk-sack free tilapia larva hatched
from spawned eggs obtained from the study of Experiment 2 were fed on the low
vitamin E diets supplemented with non-oxidized lipid (peroxide value, PV9) or
oxidized lipid (PV160) for 3 weeks. Results obtained revealed that the growth
performance of fish given the PV160 diet was poorer than larvae fed the PV9 diet but
the larvae originating from T600 diet fed parental female could to some extent alleviate
the influence of dietary lipid oxidation with the high vitamin E reserve. In conclusion,
the addition of crude palm oil or high vitamin E amount into the broodstock diet could
enhance the reproductive performance of tilapia and mother-offspring transferred
vitamin E thereafter could alleviate the negative influence of dietary lipid oxidation on
the growth performance of tilapia larvae when fed oxidized dietary lipid.
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Keywords
THE EFFECT , QUALITY