THE EFFECT OF DIETARY CRUDE PALM OIL AND ALPHA-TOCOPHERYL ACETATE ON REPRODUCTIVE PERFORMANCE AND LARVAL QUALITY OF TILAPIA (Oreochromis sp.)

dc.contributor.authorYAN, WANG
dc.date.accessioned2016-01-12T03:36:30Z
dc.date.available2016-01-12T03:36:30Z
dc.date.issued2014-09
dc.description.abstractThe present dissertation was conducted to investigate effects of the broodstock dietary lipids and vitamin E on the reproductive performance of tilapia and the quality of larvae hatched. Four isonitrogenous (35% protein) and isolipidic (10%) caseinbased diets were formulated with added fish oil (FO), FO and crude palm oil (FO+CPO; 1:1), CPO or linseed oil as the lipid source in Experiment 1, and supplemented with 0 (T0), 60 (T60), 600 (T600) mg α-tocopheryal acetate, or a CPO-derived tocotrienolsrich fraction designated to provide α-tocopherol at a level of 60 (PalmE60) mg per kg dry diet in Experiment 2. Pre-spawning female tilapia (Oreochromis sp.) was individually color-tagged, and six females and two males were stocked into a one-ton breeding tank. Each diet was fed to two tanks of broodfish and the reproductive performance of 12 individual female fish was monitored over 25 and 12 weeks, respectively. Female broodfish fed the two crude palm oil-based diets and the 600 mg α-tocopheryal acetate/kg added diet, showed significantly (P<0.05) larger ovary sizes, had the highest number of actively spawning tilapia and produced the highest total number of eggs per fish due to the shorter inter spawning interval and higher spawning frequency. The hatchability of eggs originated from these dietary treatments was also significantly higher. The inclusion of various dietary lipid source significantly affected the fatty acid composition or vitamin E concentration of muscle, liver, ovary, testes, egg and newly hatched larvae. In Expeirment 3, yolk-sack free tilapia larva hatched from spawned eggs obtained from the study of Experiment 2 were fed on the low vitamin E diets supplemented with non-oxidized lipid (peroxide value, PV9) or oxidized lipid (PV160) for 3 weeks. Results obtained revealed that the growth performance of fish given the PV160 diet was poorer than larvae fed the PV9 diet but the larvae originating from T600 diet fed parental female could to some extent alleviate the influence of dietary lipid oxidation with the high vitamin E reserve. In conclusion, the addition of crude palm oil or high vitamin E amount into the broodstock diet could enhance the reproductive performance of tilapia and mother-offspring transferred vitamin E thereafter could alleviate the negative influence of dietary lipid oxidation on the growth performance of tilapia larvae when fed oxidized dietary lipid.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1413
dc.subjectTHE EFFECTen_US
dc.subjectQUALITYen_US
dc.titleTHE EFFECT OF DIETARY CRUDE PALM OIL AND ALPHA-TOCOPHERYL ACETATE ON REPRODUCTIVE PERFORMANCE AND LARVAL QUALITY OF TILAPIA (Oreochromis sp.)en_US
dc.typeThesisen_US
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