Human molar dentinal tubules and its odontoblast process. A combined investigation using variable pressure scanning electron microscope and image analysis.
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Date
2004
Authors
Ghazali, Farid Che
Noor, Syed Hatim
Asan, Jamaruddin Mat
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
Abstract
Electron microscopy studies of the dentinal tubules are mainly investigated using
the high vacuum conventional scanning electron microscope (SEM). We aimed
to investigate its morphology using the variable pressure SEM Three almost
caries free intact adult human molar tooth were prepared as unembedded
demineralised whole dentine preparations that were initially chemically fixed with
1 Oo/o formalin for 24 hours. They were demineralised in 1 0°/o EDTA solution for 4
weeks following, which they were than section into two equal halves. The
uncoated partially demineralised dentine half tissue was inspected systematically
under a LEO VPSEM using the peltier cooling sub stage at 15 Pascal pressure
with an accelerating voltage of 15 kV. An occlusal and longitudinal observation
was made from its occlusal surface to the pulpal border. Both low and high
magnifications images were prepared from each specimen observed.
Description
Keywords
Dentine , Dentinal tubules