Human molar dentinal tubules and its odontoblast process. A combined investigation using variable pressure scanning electron microscope and image analysis.

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dc.contributor.authorGhazali, Farid Che
dc.contributor.authorNoor, Syed Hatim
dc.contributor.authorAsan, Jamaruddin Mat
dc.date.accessioned2019-10-10T03:50:08Z
dc.date.available2019-10-10T03:50:08Z
dc.date.issued2004
dc.description.abstractElectron microscopy studies of the dentinal tubules are mainly investigated using the high vacuum conventional scanning electron microscope (SEM). We aimed to investigate its morphology using the variable pressure SEM Three almost caries free intact adult human molar tooth were prepared as unembedded demineralised whole dentine preparations that were initially chemically fixed with 1 Oo/o formalin for 24 hours. They were demineralised in 1 0°/o EDTA solution for 4 weeks following, which they were than section into two equal halves. The uncoated partially demineralised dentine half tissue was inspected systematically under a LEO VPSEM using the peltier cooling sub stage at 15 Pascal pressure with an accelerating voltage of 15 kV. An occlusal and longitudinal observation was made from its occlusal surface to the pulpal border. Both low and high magnifications images were prepared from each specimen observed.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8984
dc.language.isoenen_US
dc.publisherPusat Pengajian Sains Perubatan, Universiti Sains Malaysiaen_US
dc.subjectDentineen_US
dc.subjectDentinal tubulesen_US
dc.titleHuman molar dentinal tubules and its odontoblast process. A combined investigation using variable pressure scanning electron microscope and image analysis.en_US
dc.typeArticleen_US
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