Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro
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Date
2020-11
Authors
Nithya Ravichantar
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Although HAART effectively suppresses HIV-1 replication, its compromised
effectiveness against non-B subtypes, the challenge of eliminating latent proviruses,
life-long treatment, and viral resistance complicates the cure for HIV-1.
CRISPR/Cas9, the latest genome editing tool, can overcome the limitations seen with
HAART. By targeting the promoter of HIV-1 (LTR), CRISPR/Cas9 can disrupt the
latent reservoirs. Here, we designed an improved CRISPR/Cas9 system, combinatorial
CRISPR/Cas9 to concurrently knockdown multiple HIV-1 genes: structural (Pol and
Gag), regulatory (Rev and Tat) and accessory genes (Vif). We investigated the efficacy
of this tool as a therapy against different viral subtypes in a subset population . Our
preliminary data, showed a huge decrease in viral load and we verified the safety of
CRISPR/Cas9 in human cell lines.
Description
Keywords
Combinatorial Crispr/Cas9 , Suppression Of Latent Hiv-1 Provirus In Vitro