Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro

dc.contributor.author	Nithya Ravichantar
dc.date.accessioned	2022-03-31T07:07:23Z
dc.date.available	2022-03-31T07:07:23Z
dc.date.issued	2020-11
dc.description.abstract	Although HAART effectively suppresses HIV-1 replication, its compromised effectiveness against non-B subtypes, the challenge of eliminating latent proviruses, life-long treatment, and viral resistance complicates the cure for HIV-1. CRISPR/Cas9, the latest genome editing tool, can overcome the limitations seen with HAART. By targeting the promoter of HIV-1 (LTR), CRISPR/Cas9 can disrupt the latent reservoirs. Here, we designed an improved CRISPR/Cas9 system, combinatorial CRISPR/Cas9 to concurrently knockdown multiple HIV-1 genes: structural (Pol and Gag), regulatory (Rev and Tat) and accessory genes (Vif). We investigated the efficacy of this tool as a therapy against different viral subtypes in a subset population . Our preliminary data, showed a huge decrease in viral load and we verified the safety of CRISPR/Cas9 in human cell lines.	en_US
dc.identifier.uri	http://hdl.handle.net/123456789/15005
dc.publisher	Universiti Sains Malaysia	en_US
dc.subject	Combinatorial Crispr/Cas9	en_US
dc.subject	Suppression Of Latent Hiv-1 Provirus In Vitro	en_US
dc.title	Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro	en_US
dc.type	Thesis	en_US

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