ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGE FROM RAW SEWAGE SPECIFIC FOR Escherichia coli O157:H7
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Date
2014-09
Authors
JUHARUL ZAMAN, SITI FARIZA
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Abstract
E. coli O157:H7-specific phage was successfully isolated for the first time in
Malaysia, from a sewage facility of Universiti Sains Malaysia campus in Penang.
Based on morphological study, the isolated phage was suggested to be a T4-like
phage belonging to Myoviridae family; similar to other E. coli O157:H7-specific
phages previously isolated. Physicochemical properties of the isolated phage indicate
infective (able to replicate) at temperature range from 10 °C to 37 °C, pH range from
pH 5 to pH 10 and salt concentration range from 0.17 M to 0.3 M. The isolated E.
coli O157:H7-specific phage had a narrow host range as it was able to infect only one
strain of E. coli (E. coli ATCC 13706), out of twelve different bacteria
(Enterobacteriaceae and non-Enterobacteriaceae) tested. Partial genomic studies
demonstrated high degree of identity sharing with Enterobacteria phage RB69 and
HX01 which was isolated from raw sewage in the U.S. and duck faeces in China,
respectively. The host for both phages are non E. coli O157:H7 which is E. coli B
strain for RB69 and avian pathogenic E. coli (APEC) strains, for HX01. Further
genomic comparison between the isolated phage and RB69 (similar with most of
clone sequences) showed different restriction enzyme pattern profiling though both
of them are T4-like phage in the same family, Myoviridae. Besides, partial protein
analysis revealed that the isolated phage displayed distinctive protein profile
compared with phage T4 and T7. Hence, this study provides a potential addition to
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the growing number of phages discovered, specifically E. coli O157:H7-specific
phages from raw sewage from Malaysia. The studies on its characterizations may
provide knowledge that could be useful for the development of bio-control agent
against E. coli O157:H7.
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Keywords
ISOLATION AND CHARACTERIZATION , BACTERIOPHAGE