ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGE FROM RAW SEWAGE SPECIFIC FOR Escherichia coli O157:H7
| dc.contributor.author | JUHARUL ZAMAN, SITI FARIZA | |
| dc.date.accessioned | 2016-01-12T03:32:28Z | |
| dc.date.available | 2016-01-12T03:32:28Z | |
| dc.date.issued | 2014-09 | |
| dc.description.abstract | E. coli O157:H7-specific phage was successfully isolated for the first time in Malaysia, from a sewage facility of Universiti Sains Malaysia campus in Penang. Based on morphological study, the isolated phage was suggested to be a T4-like phage belonging to Myoviridae family; similar to other E. coli O157:H7-specific phages previously isolated. Physicochemical properties of the isolated phage indicate infective (able to replicate) at temperature range from 10 °C to 37 °C, pH range from pH 5 to pH 10 and salt concentration range from 0.17 M to 0.3 M. The isolated E. coli O157:H7-specific phage had a narrow host range as it was able to infect only one strain of E. coli (E. coli ATCC 13706), out of twelve different bacteria (Enterobacteriaceae and non-Enterobacteriaceae) tested. Partial genomic studies demonstrated high degree of identity sharing with Enterobacteria phage RB69 and HX01 which was isolated from raw sewage in the U.S. and duck faeces in China, respectively. The host for both phages are non E. coli O157:H7 which is E. coli B strain for RB69 and avian pathogenic E. coli (APEC) strains, for HX01. Further genomic comparison between the isolated phage and RB69 (similar with most of clone sequences) showed different restriction enzyme pattern profiling though both of them are T4-like phage in the same family, Myoviridae. Besides, partial protein analysis revealed that the isolated phage displayed distinctive protein profile compared with phage T4 and T7. Hence, this study provides a potential addition to xx the growing number of phages discovered, specifically E. coli O157:H7-specific phages from raw sewage from Malaysia. The studies on its characterizations may provide knowledge that could be useful for the development of bio-control agent against E. coli O157:H7. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/1398 | |
| dc.subject | ISOLATION AND CHARACTERIZATION | en_US |
| dc.subject | BACTERIOPHAGE | en_US |
| dc.title | ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGE FROM RAW SEWAGE SPECIFIC FOR Escherichia coli O157:H7 | en_US |
| dc.type | Thesis | en_US |
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