Cardio-Protective Effect Of Standardized Extract Of Clinacanthus Nutans Lindau Against Doxorubicin-Induced Toxicity
Loading...
Date
2017-12
Authors
Esmailli, Kameh
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
The primary purpose of this thesis was to investigate cardio-protective effect of standardized leaf extract of Clinacanthus nutans (CN) against chemotherapy-induced cardio-toxicity. In the Far East, CN has been used traditionally to treat a wide range of diseases including herpes, cancer, fractures, diabetes, diuretic, jaundice, snake bites and kidney dysfunction. There are a number of CN products in market, however there is still lack of scientific evidence in terms of standardisation. Quality and safety of the plant raw material were examined using gravimetric analysis and microbial limit test (MLT). Various plant extracts (25% EW, 50% EW, 75% EW, 95% EW, W) were standardized using qualitative spectroscopic (UV, IR) and chromatographic (HPTLC) and quantitative chromatographic (HPLC) methods. Primary and secondary metabolites of CN extracts were analysed for their total flavonoids, polyphenols, tannins, proteins, polysaccharides and glycosaponins. Aqueous extract of CN (CN-W) showed the highest contents of glycosaponins and polyphenolics (45 ± 2.82 and 4.08 ± 0.25 w/w%, respectively). Vitexin, isovitexin, orientin, homoorientin, gallic acid, caffeic acid and chlorogenic acid were used as chemical markers for the analytical study of CN extracts. The results indicated that gallic acid is a major component in all CN extracts, while caffeic acid is the minor component. Determination and quantification of 17 free amino acids in CN extracts was done by HPLC method. The results showed that L-alanine with 3.05 ± 0.09 nmol/mg was the major free amino acid and L-glutamic with 0.66 ± 0.04 nmol/mg was the minor free amino acid in CN-W extract. Stigmasterol was isolated and characterized from methanolic leaf extract of CN.
Description
Keywords
Cardio-protective effect of standardized leaf extract , Clinacanthus nutans against chemotherapy