Cardio-Protective Effect Of Standardized Extract Of Clinacanthus Nutans Lindau Against Doxorubicin-Induced Toxicity

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dc.contributor.authorEsmailli, Kameh
dc.date.accessioned2019-04-22T01:12:24Z
dc.date.available2019-04-22T01:12:24Z
dc.date.issued2017-12
dc.description.abstractThe primary purpose of this thesis was to investigate cardio-protective effect of standardized leaf extract of Clinacanthus nutans (CN) against chemotherapy-induced cardio-toxicity. In the Far East, CN has been used traditionally to treat a wide range of diseases including herpes, cancer, fractures, diabetes, diuretic, jaundice, snake bites and kidney dysfunction. There are a number of CN products in market, however there is still lack of scientific evidence in terms of standardisation. Quality and safety of the plant raw material were examined using gravimetric analysis and microbial limit test (MLT). Various plant extracts (25% EW, 50% EW, 75% EW, 95% EW, W) were standardized using qualitative spectroscopic (UV, IR) and chromatographic (HPTLC) and quantitative chromatographic (HPLC) methods. Primary and secondary metabolites of CN extracts were analysed for their total flavonoids, polyphenols, tannins, proteins, polysaccharides and glycosaponins. Aqueous extract of CN (CN-W) showed the highest contents of glycosaponins and polyphenolics (45 ± 2.82 and 4.08 ± 0.25 w/w%, respectively). Vitexin, isovitexin, orientin, homoorientin, gallic acid, caffeic acid and chlorogenic acid were used as chemical markers for the analytical study of CN extracts. The results indicated that gallic acid is a major component in all CN extracts, while caffeic acid is the minor component. Determination and quantification of 17 free amino acids in CN extracts was done by HPLC method. The results showed that L-alanine with 3.05 ± 0.09 nmol/mg was the major free amino acid and L-glutamic with 0.66 ± 0.04 nmol/mg was the minor free amino acid in CN-W extract. Stigmasterol was isolated and characterized from methanolic leaf extract of CN.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8066
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectCardio-protective effect of standardized leaf extracten_US
dc.subjectClinacanthus nutans against chemotherapyen_US
dc.titleCardio-Protective Effect Of Standardized Extract Of Clinacanthus Nutans Lindau Against Doxorubicin-Induced Toxicityen_US
dc.typeThesisen_US
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